YEAST STRAINS PRODUCING CELLULOLYTIC ENZYMES AND METHODS AND MEANS FOR CONSTRUCTING THEM

摘要

YEAST STRAINS PRODUCING CELLULOLYTIC ENZYMES AND METHODS AND MEANS FOR CONSTRUCTING THEM Described are methods and means for the construction of strains of yeast capable of producing cellulolytic enzymes. This is achieved by the transfer of chromosomal genes or cDNA copies of mRNAs coding for cellulolytic enzymes isolated from the fungus Trichoderma reesei to yeast cells using recombinant DNA vectors capable of replicating in yeast. The correct expression of these cellulolytic genes in yeast leads to the production of cellulolytic enzymes which are secreted from the cell. This allows the yeast to hydrolyze .beta.-1,4-glucan substrates such as cellulose.