Method of expression of genes under nitrogen deficiency - pref. using the glutamine synthetase promoter 2 of E.coli and ammonium and/or glutamine as limiting nitrogen source

摘要

Method for the expression of recombinant structural genes in E.coli under the control of A promoter that is regulatable by a nitrogen source, the cells being fermented under nitrogen deficiency conditions. Pref., the promoter is the glutamine synthetase promoter 2 of E.coli (glnAP2) or DNA of similar length (+/- 10%) hybridising at 1 M NaCl at 50 deg.C with it. The E.coli strain, pref. TG1, in at least part of its growth phase is subjected to nitrogen deficiency, wherein the limiting nitrogen source may be ammonium and/or glutamine. Also claimed is an expression vector of the above features and E.coli contg. such a vector. USE/ADVANTAGE - In contrast to the prior art the vector described allows a cheap and simple induction of the promoter which is partic. useful in large scale fermenters used in biotechnolog