| 摘要 |
The invention provides novel phosphites represented by the general formula (I) and nucleoside-3'-phosphite derivatives represented by the general formula (III). (RO)3P (I) wherein R is, for example, a fluoroalkyl group or a substituted phenyl group. <IMAGE> (III) wherein R is the same as in (I), R'' is a protecting group such as dimethoxytrityl group, and B represents a base, e.g. thymine. A phosphite (I) is prepared by reacting an alcohol ROH with PCl3 in the presence of a tertiary amine, and a nucleoside-3'-phosphite (III) is prepared by reacting a phosphite (I) with a nucleoside in a solvent in the presence of a tertiary amine. Phosphites (I) are very stable. Using a nucleoside-3'-phosphite (III) an oligonucleotide can be synthesized on a solid support by a simplified process.
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