发明名称 |
METHOD FOR EXTRACTION OF INTRACELLULAR COMPONENTS |
摘要 |
A method of preparing an extract of an intracellular component, wherein cells are contacted with an extractant to generate an extract solution, is characterised by contacting the solution with a cyclodextrin to neutralise the extractant. Preferred extractants are cationic or other kinds of surfactants. The cyclodextrin is preferably used in solution. Preferred intracellular components are ATP which can, after neutralisation of the extractant, be assayed by a firefly luciferase assay; and DNA or RNA which can, after neutralisation of the surfactant, be amplified or further processed in other ways. |
申请公布号 |
WO9212253(A1) |
申请公布日期 |
1992.07.23 |
申请号 |
WO1992GB00056 |
申请日期 |
1992.01.10 |
申请人 |
AMERSHAM INTERNATIONAL PLC |
发明人 |
LUNDIN, ARNE;ANSON, JOHN, GEORGE |
分类号 |
C07H1/08;C07H19/20;C12Q1/00;C12Q1/66;C12Q1/68 |
主分类号 |
C07H1/08 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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