摘要 |
PURPOSE:To easily obtain a polysaccharide having stable constituent component ratio at a low cost by culturing a bacterial strain of genus Alcaligenes on a synthetic medium. CONSTITUTION:A synthetic medium (A) is produced by compounding 1-4wt.% of one or more sugars selected from glucose, fructose, maltose, etc., 0.01-0.1wt.% of one or more inorganic salts selected from K or Na phosphate, sodium chloride, ammonium chloride, etc., 10-1,000mg/l of one or more amino acids selected from Arg, Cit, Orn, Vat, etc., and 0.03-10mg/l of one or more minor metal salts selected from sulfate, chloride, etc., of Fe, Ca, Mn, etc. Alcaligenes latus B-16 strain (FERM BP-2015) (B) is inoculated to the medium A and aerobically cultured at 15-40 deg.C and pH4-10 for 1-10 days under aeration and agitation in deep tank to obtain a cultured product (C). The objective polysaccharide having water-absorptivity, moisture-absorptivity, moisture retention and viscosity is produced by treating the component C by ethanol precipitation process, etc. |