摘要 |
NEW MATERIAL:The manifestation vector containing a manifestation unit consisting of effectively linked yeast PH05 promoter, structural gene coding human Mn SOD and an yeast terminator, an yeast replicator, an yeast Leu2d selection marker, an E.coli replicator and an E.coli selection marker and enabling the induced manifestation of human Mn SOD with an yeast. EXAMPLE:Plasmid pYMT1-SOD21. USE:Production of active human Mn SOD, etc. PREPARATION:The exemplified plasmid can be produced by the process shown in the figure. |