发明名称 |
Non-corrosive compositions and methods useful for the extraction of nucleic acids |
摘要 |
This invention relates to safe and effective methods for the extraction of nucleic acids. In particular, methods are described for isolating nucleic acid from a sample containing a biological mixture of nucleic acids and other biological compounds wherein the sample is combined with an extraction solution containing at least one organic compound such as benzyl alcohol or a benzyl alcohol derivative to form an aqueous and non-aqueous phase. The nucleic acid is isolated from the aqueous phase. Preferably, the resulting combined solution also contains bentonite, as defined below. Typically, the sample will first be combined with a lysing agent before extraction. The lysing agents preferred are chaotropic salts such as guanidinium hydrochloride and guanidinium isothiocyanate.
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申请公布号 |
US5130423(A) |
申请公布日期 |
1992.07.14 |
申请号 |
US19910649389 |
申请日期 |
1991.02.01 |
申请人 |
MICROPROBE CORPORATION |
发明人 |
VAN NESS, JEFFREY;CIMLER, B. MELINA;MEYER, JR., RICH B.;VERMEULEN, NICOLAAS M. J. |
分类号 |
C12N15/10;C12Q1/68 |
主分类号 |
C12N15/10 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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