发明名称 |
PURIFICATION OF RECOMBINANT HEPATITIS B SURFACE ANTIGEN |
摘要 |
PURIFICATION OF RECOMBINANT HEPATITIS B SURFACE ANTIGEN Yeast proteins hydrophobically bonded to a desired protein or polypeptide produced in a transformed yeast are removed by treating the antigen with a chaotropic agent and concentrating the treated antigen in a hollow fiber capillary bundle having a molecular weight cutoff greater than 50,000 daltons but less than 1,000,000 daltons. The concentrated antigen is then diafiltered using a chaotropic agent to remove substantially all remaining yeast protein. |
申请公布号 |
CA1304887(C) |
申请公布日期 |
1992.07.07 |
申请号 |
CA19880560864 |
申请日期 |
1988.03.08 |
申请人 |
MERCK & CO., INC. |
发明人 |
HURNI, WILLIAM M.;KUBEK, DENNIS J.;MILLER, WILLIAM J.;RIENSTRA, MARK S.;SCOLNICK, EDWARD M. |
分类号 |
C12P21/00;A61K39/29;C07K1/34;C07K14/02;C07K14/10;C07K14/18 |
主分类号 |
C12P21/00 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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