16SRRNA GENE OF BORDETELLA BRONCHISEPTICA, DNA FRAGMENT FOR PROBE AND METHOD FOR DETECTING BORDETELLA BRONCHISEPTICA USING THE SAME

摘要

NEW MATERIAL:The subject gene having a base sequence expressed by the formula. USE:Detection of Bordetella bronchiseptica, etc. PREPARATION:A DNA of Bordetella bronchiseptica is cleaved with BamHI and inserted into lambda phage vector EMBL4 to prepare a DNA library, which is then cloned by a polymerase chain reaction (PCR) method to provide a 16 SrRNA genetic fragment. The resultant genetic fragment is subsequently used as a probe to carry out screening. A alone containing 16SrRNA gene of a complete length is selected. A BamHI-SalI DNA fragment of about 1.7kb containing the 16SrRNA gene of the complete length is obtained from the prepared clone.