| 摘要 |
<p>The invention relates to a method of determining a ligand in a liquid sample by bringing the sample into contact with a vibrating object which is accommodated in an acoustic measuring device and whose surface is provided with specific binding partners for the ligand to be determined, and analysing the frequency shift or the change in the acoustic propagation velocity or the change in the amplitude or the phase change measured at the vibrating object and due to the formation of a complex between the ligand and the specific binding partner, which method is characterised in that a vibrating object is used whose surface situated at the liquid/solid interface is provided with either a multiplicity of latex spherules having a diameter of 50-1000 nm as particulate carriers which contains specific binding partners for the ligand; or a multiplicity of specific binding partners which are provided with a 'leaving agent' in the form of a particle reversibly bound to said specific binding partners and being a ligand on a latex spherule having a diameter of 50-1000 nm; or a multiplicity of specific binding partners for the ligand which are partly provided with the ligand and the 'leaving agent' in the form of a ligand on a latex spherule having a diameter of 50-1000 nm, which ligand is reversibly bound to said specific binding partners in accordance with an established equilibrium with a medium (competition reaction) which contains the ligand and the 'leaving agent', said established equilibrium being temporarily shifted after introducing a sample to be examined resulting in a corresponding frequency shift.</p> |
