| 摘要 |
<p>Immortalized cell lines preserving the same properties as those of differentiated cells or primary precursors of the original donor, applications thereof as a system for the production of differentiated cells in large numbers and model for studying the physiopathology of the differentiated cells obtained. Application of said differentiated cells and/or precursors as an agent serving a preventive and/or therapeutic function, especially in the treatment of genetic diseases, and more particularly, myopathies. Said immortalized cell lines are obtained by appropriately transforming suitable primary cells, derived from the organ of a suitable animal such as a mammal or a bird, with a nucleic acid fragment comprising a suitable immortalizing viral oncoqene, at least one fragment of the regulating zones of the vimentin human gene and optionally a gene lacking its promotor whose activity is readily detectable either by using a simple enzymatic test or because it imparts resistance to an antibiotic.</p> |
