| 摘要 |
<p>B-cell lymphomas reveal surface immunoglobin (immunoglobin) containing unique idiotypic (idiotype) determiners which can be exploited as specialised tumour markers. The subject has produced muscular monoclonal antibodies (MAbs) in reaction to the idiotype marker, produced by 67 patients with small cleaved cell, low level lymphomas. Except for the, 199 monoclonal cleaved cell antibodies, 47 (24 percent) were found to react to immunoglobin group normal human serum at concentrations ranging from 0.6μg/ml to 160μg/ml (micrograms/cc.) Of these 40 monoclonal antibodies, 90 percent cross-reacted with an idiotype produced in normal serum at levels lower than 50μg/ml. Thirty two of these anti-idiotypes were directed toward the shared idiotype, expressed in other lymphoma cells of patients. The frequent expression of shared idiotypes was determined by each antibody ranging from 0.26 percent to 3.9 percent of tested B-cell lymphomas. A group of five anti-idiotype antibodies reacted with 80 percent of lymphomas associated with AIDS. Based on reactivity with these monoclonal antibodies, tumours could be classified into specific families. Globally, the 32 monoclonal antibodies reacted with the entire 108 of 332 B-cell lymphoma cases (32.5 percent) including 35 of 116 small cleaved cell type lymphomas (30 percent). Many of these anti-shared idiotypes reacted with more than one histopathological lymphoma subtype. Anti-idiotypes have been used in diagnosis and therapy of B-cell lymphomas. The subject additionally revealed at least seven anti-shared idiotype antibodies reacting with antibodies, 16.6 and RF, for example. Development of a library of anti-idiotypes reactive with shared idiotypes will facilitate these clinical studies, obviating the need to culture the usual hybridomas for each patient.</p> |
