发明名称 |
EXPRESSION SYSTEMS |
摘要 |
Expression systems which comprise a mammalian host, such as erythroid cells, transformed with a vector which comprises a promoter, a DNA sequence which codes for a desired polypeptide and a dominant control region. The vectors and methods for preparing polypeptides using the expression systems are also described. In a preferred embodiment, the vector contains cDNA for the desired polypeptide and a sequence which is capable of stabilising mRNA produced from the cDNA. The expression systems are particularly efficient and are capable of secreting the desired polypeptide.
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申请公布号 |
CA2058280(A1) |
申请公布日期 |
1992.06.22 |
申请号 |
CA19912058280 |
申请日期 |
1991.12.20 |
申请人 |
IMPERIAL CHEMICAL INDUSTRIES PLC |
发明人 |
HOLLIS, MELVYN;NEEDHAM, MAURICE, R. C.;GOODING, CLARE;GROSVELD, FRANKLIN G.;ANTONIOU, MICHAEL |
分类号 |
C12N5/10;C07K14/47;C07K14/61;C07K14/715;C07K14/72;C07K14/765;C07K14/805;C12N9/20;C12N15/09;C12N15/12;C12N15/85;C12P21/00;C12P21/02;C12R1/91;(IPC1-7):C12N15/85;C12P21/08;C12N15/64 |
主分类号 |
C12N5/10 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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