| 摘要 |
Carotenoids and aminosyndia are coproduced by fermenting Streptomyces chrestomyceticus var aurantioidus (722 I.P.V. Milan University) in submerged culture under aerobic conditions in an aqueous nutritive medium containing a source of carbon, nitrogen and mineral salts and mycelium, containing carotenoids, is separated from the fermentation broth, containing aminosydin, by filtration or centrifugation. Carotenoid yields are increased by strong aeration (0.8 to 1.0 litres of air per minute per litre of culture and 400-500 r.p.m. agitation) and media in which the carbon souce is glucose, dextrin, lactose or mannitol. Aminosyndin yields are increased by slight aeration (0.5 to 0.8 litres of air per minute per litre of culture and 300-400 r.p.m. agitation) and media in which the carbon source is starch. A medium containing a mixture of cottonseed oil and soyabean oil is particularly suited to coproduction of these substances. A typical medium consists of starch, casein, magnesium sulphate, potassium hydrogen phosphate, ferrous sulphate, zinc sulphate, copper sulphate and water. A temperature of 24 to 32 DEG C., a pH of 5 to 8 and cultivation period of 1 to 8 days is employed. A two-stage process, in which the micro-organism is cultivated under conditions specially suitable for the production of (a) aminosydin and then (b) carotenoids may be used, the mycelium separated from the first stage (a) being used in the second stage (b). Aminosydin may be recovered from the clarified fermentation broth by conventional methods as in Specification 880,035. The carotenoids may be obtained by extracting the mycelium with an organic solvent such as chloroform, acetone or methylene dichloride. The separation of carotenes from xanthophylls may be by adsorption or magnesium oxide, siliceous earth or alumina. Streptomyces chrestomyceticus var. aurantioidens is prepared by treating S. chesetomyceticus NC:B 8995 with a phosphate buffer solution of N-nitroso-N-methyl-urethane. |
