| 摘要 |
<p>PURPOSE:To simply obtain the title bile acid of high purity by reaction of taurine with a bile acid followed by removing the organic solvent or unreacted raw materials and then by injecting the resulting aqueous solution into a column packed with e.g. ODS silica gel followed by elution with e.g. an organic solvent. CONSTITUTION:A bile acid of formula I (R<1> to R<4> are each H, alpha- or beta-hydroxyl group which may carry a protecting group, or ketone) is reacted with taurine, and a liquor after reaction is feed from the organic solvent or unreacted raw materials, and the resulting aqueous solution is injected into a column packed with 2-20 times (v/w) reverse-phase synthetic resin or ODS silica gel based on the taurine-conjugation-type bile acid followed by elution with a water-soluble organic solvent (e.g. methanol) singly or its mixture with water, thus obtaining the objective compound of formula II (X is H or alkali metal).</p> |
