| 摘要 |
This invention relates to a method for provoking a restricted T cell response, and for identifying agents which are capable of causing such a response. Essentially, peptide fragments are contacted to a cell free expression medium containing nucleic acids sequences which code for or express the components of an MHC or HLA antigen. By adding test peptides to the cell free medium and then determining the extent of assembly of the components to form a complete antigen molecule, it is possible to identify substances which provoke restricted T cell responses. Also described is a method by which MHC or HLA antigens and the cells which present them can be assayed, using a labelled peptide specific for the antigen, and a binding partner to the label. |
