Liq. chromatographic system for determining glycosylated forms of haemoglobin - including sepn. column, automatic sampler, eluent selection system and control elements

摘要

Liq. chromatography system comprises (1) device for taking a sample and supplying it, as well as least one eluent, to at least one sepn. column; (2) device for supplying eluent; (3) device for detecting components separated on the column, and (4) control system for (a) calculating the component ratios and other parameters of the detected components, (b) detecting the operational conditions of the working elements of the system and (c) forwarding signals to these working elements. The column is packed with an organic porous material carrying functional (esp. carboxyalkyl) gps. and in the dry state has pore dia. 6-120mm; ion exchange capacity 0.1-0.5 meq/g; particle size 3-4 microns and pore vol. 0.2-0.6 ml/g. USE/ADVANTAGE - The system is used to analyse biological and living compounds, in blood etc. esp. glycosylated forms of haemoglobulin (Hb) to monitor the long term glucose status of diabetics. It allows analysis of all 5 or 6 Hb components an dprovides rapid sepn. of the stable glycosylated material (SA1c) from others.