Sepn. of microorganism exo-cellular protein, esp. for enzyme - by addn. of adsorbent to remove substances hindering protein pptn., concn., and pptn. of protein

摘要

Exocellular proteins from microorganisms are sepd. from a filtered fermentation broth by (a) removing substances which hinder protein pptn., with a solid adsorbent, (b) concentrating the remaining soln. to 30-40 wt.% protein content, (c) allowing the proteins to ppte. at pH 6-10, and sepg., opt. with addn. of a precipitant for proteins to accelerate the pptn.