| 摘要 |
The detection of antibodies in a test sample is carried out by: (a) Preparing an essentially isotonic and low ionic strength suspension comprising said sample and erythrocytes in net negatively-charged form; (b) maintaining said suspension for at least 30 seconds; (c) combining said suspension with an amount of a solution of polymer effective for agglutination of said erythrocytes; (d) separating the resultant agglutinates of polymer and erythrocytes from supernatant of said suspension; (e) dispersing said agglutinates in a hypertonic salt solution having an essentially neutral pH; and (f) monitoring the dispersed agglutinates for dissocation of erythrocytes. Antibody presence is indicated by persistence of the agglutinates in the dispersion resulting from step (e). The antigen which reacts with the antibody may be native to the erythrocytes, or may be unrelated to the erythrocytes by synthetically coupled to these red blood cells. Quantitative assay is also realizable because the degree of agglutinate dissociation is inversely related to antibody concentration in the sample. |
