| 摘要 |
PURPOSE:To improve the productivity of guanosine, by adding a vector integrated with a genetic field of purine analogue resistance or dechoinine resistance to a variant belonging to the genus Bacillus, having adenine demanding properties. CONSTITUTION:Chromosome DNA taking part in purine analogue resistance or dechoinine resistance is extracted from variant belonging to the genus Bacillus, having purine analogue resistance or dechoinine resistance. The chromosome DNA is connected to vector DNA, and the prepared recombined vector DNA integrated with a chromosome DNA piece is introduced to a variant belonging to the genus Bacillus, having adenine demanding properties. The transformed substance thus obtained is cultivated in a liquid medium containing a carbon source, nitrogen source, inorganic salt, adenine, etc., and guanosine is accumulated in the culture. |
