TWO-LAYERED GELATIN AND METHOD FOR PREPARING THE SAME

摘要

PURPOSE:To provide a crosslinked gelatin sheet having improved cell-affinity and bio-compatibility at a low cost by forming one side of the sheet in a dense thin film state and the other side in a wall surface state having opened holes. CONSTITUTION:An aqueous solution containing 1-20wt.% (based on gelatin) of a crosslinking agent such as glycerol polyglycidyl ether and approximately 10wt.% of purified gelatin obtained from cattle bones, etc., is cast in a plate-like mold frame whose solution-contacting surface is produced of a fluororesin and whose other surface is opened. The cast solution is horizontally held so as to form a sheet and subsequently cooled at 0-20 deg.C for >=2hr so that one side of the sheet is maintained below a freezing temperature and the other side further above the freezing temperature to form a temperature gradient gradually rising from one side to the other surface. The gelled and frozen product is subsequently vacuum-lyophilized at <=30 deg.C and heat-treated at 80-150 deg.C within 10hr to prepare a two-layered gelatin sheet having a thickness of <=200mum and comprising a dense thin film 1 without or with pores of <=1mum and a wall surface 3 having opened holes 4 of >=50mum on average.