Xyloseisomerase gene of thermus aquaticus, xyloseisomerase and process for preparation of fructose.

摘要

A xyloseisomerase gene from Therumus bacteria, such as Therumus aquatics (ATCC27634) and a gene having 60 % or more of homology to the amino acid sequence of Therumus aquatics xyloseisomerase gene. A xyloseisomerase from Therumus aquatics characterized in that the xyloseisomerase has the optimal pH of about 7, the stable pH range of from about 6 to 8.5, the optimal temperature of about 95 DEG C and the molecular wieght of about 44000, and is stabilized with manganese or magnesium. A process for preparing a xyloseisomerase comprising transforming a microorganism with a plasmid containing the above gene and a promotor, culturing the transformed microorganism and harvesting the produced xyloseisomerase. A process for preparing fructose comprising isomerization of glucose to fructose in the presence of the above xyloseisomerase.