PROCESS FOR PRODUCING BULL GROWTH HORMONE (BGH) FROM YEAST BY RECOMBINANT DNA

摘要

A method for mass producing cattle growth hormone (CGH) comprises: (a) synthesising oligonucleotides having SacI, PstI and SalI restriction sites according to the amino acid sequence of CGH; (b) cloning the fragment to the E.coli vector by ligating to obtain the CGH gene; (c) ligating the obtd. CGH gene and artificial synthetic linker into the E.coli vector to make the CGH gene joined with the linker; (d) re-cloning the CGH gene joined with the linker into a E.coli expression vector having a trp-promotor; (e) expressing the resultant in E.coli.