发明名称 |
METHOD OF PRODUCING PEPTIDES USING BACULOVIRUS VECTORS IN CULTURED CELLS |
摘要 |
Recombinant Bombyx mori nuclear polyhedrosis viruses having, in the polyhedral protein-encoding structural gene portion of the Bombyx mori nuclear polyhedrosis virus DNA (BmNPV DNA), a structural gene for a desired protein as joined to the whole of or a part of the polyhedral protein-encoding structural gene with or without interposition of a linker base sequence are provided. By propagating the above viruses in silkworm-derived cells or silkworms, fused proteins of a desired protein and the whole of or a part of the polyhedral protein as joined together with or without interposition of a linking amino acid or peptide are produced. The desired proteins can be obtained from the fused proteins by cleavage or decomposition at the linking site.
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申请公布号 |
US5110729(A) |
申请公布日期 |
1992.05.05 |
申请号 |
US19910641795 |
申请日期 |
1991.01.16 |
申请人 |
DAIICHI SEIYAKU CO., LTD. |
发明人 |
MAEDA, SUSUMU;FURUSAWA, MITSURU;MARUMOTO, YASUMASA;HORIUCHI, TADASHI;SATO, YOSHINARI;SAEKI, YOSHIYUKI |
分类号 |
C12N15/09;C07K14/01;C07K14/56;C07K14/65;C12N7/00;C12N7/02;C12N7/04;C12N15/00;C12N15/62;C12N15/866;C12P21/00;C12P21/02;C12R1/91 |
主分类号 |
C12N15/09 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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