COLLECTION OF MUTANT OF CELL USING LASER

摘要

PURPOSE:To safely and easily obtain the subject mutant without using troublesome operations and harmful reagent by carrying out the destruction of cell and perforation of the cell wall and cell membrane in a solution with a laser beam, thereby forming a cell having characteristics different from those of original cell. CONSTITUTION:Two or more kinds of cells such as tumor cell and an immunized mouse sphlenocyte are put into the same solution in a Petri dish 1. The dish 1 is set in a laser-type cell processor 2 and irradiated with laser beam to destruct one or more kinds of cells in the solution and perforate the cell wall or cell membrane of the other one kind of cell. The intracellular substance of the destructed cell is taken into the perforated cell to effect the cell fusion. The fused cell is cultured on an HAT medium to selectively proliferate the fused hybrid cells and obtain a mutant cell consisting of a hybrid cell having proliferation performance and antibody-productivity different from those of the original cells.