发明名称 |
AMPLIFICATION OF MIDIVARIANT DNA TEMPLATES |
摘要 |
Methods are provided for the amplification of a midivariant DNA containing an inserted target specific nucleic acid sequence(s) to enable detection of the presence of a target nucleic acid sequence in a test sample. One method employs midivariant DNA as a template for the synthesis of RNA catalyzed by QB replicase. Two midivariant DNA/probe conjugates including a nonreplicable portion of midivariant DNA and a target specific nucleic acid sequence (probe) are described. An amplification method including DNA/prove conjugates followed by replication of the DNA template has enabled detection of less than 200 template molecules. In a modified amplification method one of the midivariant DNA/probe conjugates further includes a RNA polymerase promoter sequence to enable transcription of the midivariant DNA template into RNA. The sequential ligation-transcription-replication method enables the detection of a single template molecule. The detectable molecule(s) are indicative of the presence of the target nucleic acid sequences in the test sample. |
申请公布号 |
AU8045391(A) |
申请公布日期 |
1992.04.30 |
申请号 |
AU19910080453 |
申请日期 |
1991.07.15 |
申请人 |
CIBA CORNING DIAGNOSTICS CORP. |
发明人 |
RICHARD M. MARTINELLI;JEFFREY J. DONAHUE;JOHN T. UNGER |
分类号 |
C07H21/04;C12N15/09;C12Q1/68 |
主分类号 |
C07H21/04 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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