| 摘要 |
PURPOSE:To achieve a higher sample introduction accuracy eliminating sample introduction errors caused by the type of a sample by making the sample concentrated by the application of a voltage migrate in an opposite direction to a concentration means to analyze the sample. CONSTITUTION:A sample in a sample rack 18 is sucked up with a nozzle 17 and transferred to a sample injection port 10 of a sample introduction valve 5 to inject the sample and then, a rotor 8 is switched to move the sample to a closed passage between an electrode 11 and a capillary 1. Polarity of a high voltage power source 15 is adjusted so that desired components in the sample may be concentrated on the side of the electrode 11 and a specified DC voltage is applied between the sample and an electrode 25 for more than a specified time to concentrate the sample. Then, when the polarities of the electrodes 11 and 25 are inverted, the desired components move through the capillary 1 to be separated into individual components according to a speed difference of migration and reach an electrode cell 24 passing through a quartz capillary 23. At this point, light of a light source 26 passes through the capillary 23 by way of a lens 27, a spectroscopic mirror 38 and the like to detect 31 absorbance of the components. This eliminates sample introduction errors and achieves an increase in degree of concentration thereby improving analysis sensitivity. |
