| 摘要 |
<p>NEW MATERIAL:An amino acid sequence-containing protease inhibitor BbrPI of the formula. USE:A reagent for researching protease control systems, a drug and a produced protein decomposition-preventing agent in microorganism-utilizing industries. PREPARATION:For example, Bacillus brevis HPD 31 (FERM BP-1087) is cultured in a liquid medium at 30 deg.C for 3hr and centrifuged to remove the bacterium bodies. The remained supernatant is collected, mixed with ammonium sulfate so as to form a 0.8 saturation, and centrifuged. The precipitation fraction is collected, dialyzed and subjected to a gel filtration two times. An active fraction is separated, mixed with ammonium sulfate to form a 0.8 saturation, and centrifuged. The precipitates are collected, dialyzed and subjected to an ion exchange column chromatography. An active fraction is subject to a SDS-polyacrylic acid gel electrophoresis. The active band is selected and dialyzed to provide the protease inhibitor BbrPI.</p> |
