发明名称 |
STABLE DNA CONSTRUCTS FOR EXPRESSION OF ALPHA-1-ANTITRYPSIN |
摘要 |
Methods are provided for producing alpha-1-antitrypsin in host cells and for selecting transformed cells comprising the step of transforming the host cell with a DNA molecule comprising a gene which complements a deficiency in the host cell. The host cell is a strain having a deficiency in a function necessary for normal cell growth. The gene in the DNA molecule, such as a plasmid, which complements the deficiency serves as a selectable marker whereby the growth conditions for selection may comprise a conventional complex medium. |
申请公布号 |
CA1299120(C) |
申请公布日期 |
1992.04.21 |
申请号 |
CA19850493475 |
申请日期 |
1985.10.21 |
申请人 |
ZYMOGENETICS, INC.;KAWASAKI, GLENN |
发明人 |
KAWASAKI, GLENN;BELL, LESLIE R. |
分类号 |
C07K14/81;C07G99/00;C07H21/04;C07K14/00;C07K14/005;C07K14/195;C12N1/00;C12N1/16;C12N1/19;C12N1/20;C12N1/21;C12N5/00;C12N9/99;C12N15/00;C12N15/09;C12N15/15;C12N15/64;C12P19/34;C12P21/00;C12P21/02;C12R1/19;C12R1/865 |
主分类号 |
C07K14/81 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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