DIHYDROFOLATE REDUCTASE-ANTIALLERGIC PENTAPEPTIDE FUSED PROTEIN

摘要

NEW MATERIAL:A recombinant plasmid containing a base sequence encoding dihydrofolate reductase-antiallergic peptide fused protein. EXAMPLE:Plasmid pBBK10MM. USE:Production of DSDGK. PREPARATION:DNAs shown by formula II and formula III are chemically synthesized and a duplex DNA shown by formula TV is obtained by annealing. Recombinant plasmid pMEK2 is cleft with BamHI and XhoI to give a DNA fragment, which is linked to the duplex DNA shown by formula TV with T4-DNA ligase to give new plasmid pBBK10MM.