PLASMIDE SENSIBILE ALLA TEMPERATURA

摘要

A plasmid having a temperature-sensitive replication origin which has the following properties: a) capable of autonomous replication in Corynebacterium and being retained in Corynebacterium; and, b) when a cell containing said plasmid is cultured at 31 DEG to 37 DEG C., replication of the plasmid is inhibited and at the same time, the plasmid is removed from the cell body; and a method for performing homologous recombination in Corynebacterium using the plasmid.