Method for obtaining recombinant surface antigen of hepatitis B virus (HEP B) of higher immunogenic capacity and use thereof in a vaccine preparation.

摘要

A multi-step process for recovering hepatitis B surface antigen from Pichia pastoris cells containing a gene encoding said hepatitis B surface antigen and having expressed it. The process leads to a high yield of very pure, homogeneous HBsAg in a particulated form having a high immunogenicity, suitable for use in vaccine compositions for treating human beings and for use in diagnostic methods.