摘要 |
PURPOSE:To enable easy measurement of the concentration of a substance to be measured in a biosample by a method wherein an enzyme reaction layer composed of oxidoreductase, an electron acceptor and a hydrophilic macromolecule is formed on the surface of an electrode system. CONSTITUTION:An enzyme reaction layer 8 composed of oxidoreductase, a hydrophilic macromolecule and an electron acceptor is formed on the surface of an electrode system 2a, 3a made up of a measuring electrode 2 and an opposite electrode 3 on an insulative base 1. As for the oxidoreductase, glucose oxidase, alcohol oxidase, cholesterol oxidase, xanthine oxidase or the like can be used, for instance. As for the electron acceptor, potassium ferricyanide, P-benzoquinone, 2,6-dichlorophenol-indophenol, methylene blue, phenazine methosulfate, beta-naphthoquinone-4-sulfonic acid potassium, ferrocene or the like can be used. According to this constitution, a minute quantity of specific component substance in a biosample can be quantified quickly and simply. |