| 摘要 |
PURPOSE:To rapidly measure the subject number of bacteria in a plant water used in the field of semiconductor production, etc., in industrial scale by quantitatively measuring the number of bacteria in a treated water such as an ion- exchanged water or a water treated with a reverse osmosis membrane using a specified process. CONSTITUTION:Bacteria contained in an ion-exchanged water or a water treated with a membrane such as a reverse osmosis membrane, an ultrafiltration membrane or a precision filtration membrane are initially collected on a sheet (preferably filter membrane made of nitrocellulose-based fiber, etc., and having <=0.45mum pore size) having an affinity to Pyrenomycetes in a two-dimensionally dispersed state. Nucleic acids of the collected bacteria are exposed to the outside of the cell and a substance having a bonding capacity to the nucleic acids and labeled with a labeling substance such as an enzyme, a fluorescent substance and a radioactive substance is bonded to the above-exposed nucleic acids. The number of points of the labeled substance adsorbed on the sheet is subsequently counted utilizing the labeled substance as an index, thus carrying out the objective quantitative measurement. |
