| 摘要 |
POLYMERIC MATRIX FOR AFFINITY CHROMATOGRAPHY AND IMMOBILIZATION OF LIGANDS A matrix useful for immobilizing organic ligands, such as biologically active materials, is prepared by reacting a hydroxyl group containing polymer with polyethyleneimine. Specifically, the matrix of the present invention is prepared by a process which includes the steps of reacting a hydroxyl containing polymer with 2-fluoro-1-methylpyridinium toluene-4-sulfonate (FMP) to form an activated polymer wherein at least some of the hydroxyl groups of the polymer have been substituted to form 1-methyl-2-pyridoxy toluene-4-sulfonate groups, and reacting the 1-methyl-2-pyridoxy substituted polymer with polyethyleneimine to form an affinity chromatographic matrix in which at least some of the 1-methyl-2-pyridoxy groups of the polymer have been replaced by polyethyleneimine. |
