发明名称 |
EXPRESSION OF G PROTEIN COUPLED RECEPTORS IN YEAST |
摘要 |
Disclosed is a transformed yeast cell containing a first heterologous DNA sequence which codes for a mammalian G protein coupled receptor and a second heterologous DNA sequence which codes for a mammalian G protein alpha subunit (mammalian G alpha ). The first and second heterologous DNA sequences are capable of expression in the cell, but the cell is incapable of expressing an endogenous G protein alpha -subnit (yeast G alpha ). The cells are useful for screening compounds which affect the rate of dissociation of G alpha from G beta gamma in a cell. Also disclosed is a novel DNA expression vector useful for making cells as described above. The vector contains a first segment comprising at least a fragment of the extreme amino-terminal coding sequence of a yeast G protein coupled receptor. A second segment is positioned downstream from the first segment (and in correct reading frame therewith), with the second segment comprising a DNA sequence encoding a heterologous G protein coupled receptor. |
申请公布号 |
WO9205244(A1) |
申请公布日期 |
1992.04.02 |
申请号 |
WO1991US06605 |
申请日期 |
1991.09.12 |
申请人 |
DUKE UNIVERSITY |
发明人 |
KING, KLIM;DOHLMAN, HENRIK, G.;CARON, MARC, G.;LEFKOWITZ, ROBERT, J. |
分类号 |
C07K14/39;C07K14/47;C07K14/705;C12N1/18;C12N1/19;C12N15/09;C12N15/12;C12N15/74;C12N15/81;C12P21/02;C12Q1/00;C12Q1/02;C12Q1/68;C12R1/865;G01N33/566 |
主分类号 |
C07K14/39 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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