| 摘要 |
<p>A microassay card (10) includes an upper layer (12) containing wells (14) for receiving a liquid sample (16). A second layer (20) of the card (10), beneath the first layer (12), includes a supporting surface (223) bound to a reactive species (114). A third layer (26) includes a superabsorbent support (26) impregnated with an indicator (228). Typically, the indicator (228) is a substrate (228) for an enzyme (16), such as a reduced dye precursor and a source of hydrogen peroxide necessary for the action of the enzyme (116), upon the substrate to cause a spectral change in the absorbent layer (26). By selecting the structure of the first (12) and second (20) layers, the card (10) can be formatted for a displacement assay or a competitive assay. The microassay card (10) of the present invention is particularly useful for drug testing.</p> |
