Vertical transport chamber for electro-physiological epithelia study - provides exchangeable test surfaces with measurement openings as small as 0.5 mm in adjustable halves

摘要

The device consists of two flat half chambers and is suitable for adjustment under a stereo or binocular microscope while the sample is being introduced. Micro-samples in physiological or other media are placed in drops direct onto the inner sides of the half chambers. The epithelia are studied under constant conditions, and erfusion of the epitheila may be at equal or asymmetric pressures on on either side. Half-maximal mass transfer of the substances in the perfusion solution is provided ina few seconds, using substances such as inhibitors of ion transport and pH regulation; hormones; pharmaceutical/poison mixt.; substrates such as sugar, amino-acids, fats, fatty acids; and eco-toxicoiogical contaminates such as cadmium, lead etc. Reversibility, irreversibility and other kinetic parameters represented by electrophysicological quantities such as the trans-epithelial potential difference, short-circuit current, resistance etc. are measured. USE/ADVANTAGE - Study of transport of ions etc. in tissues (epithelia, cuticles) of organs and organisms. Pharmacology, eco-toxicology and transport-physiology. Allows study of smaller samples having a min. measuring surface dia. of about 0.5 mm.