PURIFICATION, PRODUCTION AND USE OF TUMOR NECROSIS FACTORS
摘要
<p>A process of producing mammalian tumor necrosis factors (TNF) and TNF-like polypeptides by culturing prokaryotic hosts transformed with a recombinant DNA molecule comprising DNA sequences encoding those polypeptides operatively linked to either one of the T4 or the pL-T4 expression control sequences. A process for purifying TNF-like polypeptides using an anion exchanger. The TNFs and TNF-like polypeptides produced by the processes of this invention, and compositions and methods utilizing those TNFs and TNF-like polypeptides, are useful in anticancer, antitumor and antimalarial therapies. They are also useful together with interferon therapy and chemotherapy in anticancer and antitumor therapies, and in combination with actinomycin D in the treatment of tumor-bearing mammals.</p>
申请公布号
EP0216786(B1)
申请公布日期
1992.03.18
申请号
EP19860900138
申请日期
1985.12.19
申请人
BIOGEN, INC.
发明人
FIERS, WALTER, C.;FRANSEN, LUCIA, M.;TAVERNIER, JAN, HONORE, LEO;MARMENOUT, ANNE, LOUIS, MARIE;VAN DER HEYDEN, JOSE;ALLET, BERNARD;KAWASHIMA, ERIC, H.