METHOD OF PRODUCING CORE STRUCTURE OF RETRO VIRUS AND PARTICLE PRODUCED THEREBY.

摘要

A method of producing a core structure of a retro virus which is constituted of cleavage type gag protein and freed from env protein and env gene either having or expressing infectivity, and a vaccine containing this structure as an active ingredient. The core structure can be obtained either by infecting an animal cell with a vaccinia virus comprising gag and pol genes of a virus belonging to the category of retro virus incorporated thereinto without incorporating any env gene, or by infecting an animal cell with both of a vaccinia virus comprising said gag gene incorporated thereinto without incorporating any env gene and another vaccinia virus comprising said pol gene incorporated thereinto without incorporating any env gene; culturing the infected animal cell; and purifying the core structure separated from a supernatant of the culture medium.