发明名称 |
Liposome for entrapping gene, liposomal preparation and process for the manufacture of the preparation. |
摘要 |
For increasing gene entrapping efficiency, it is preferable that liposome membrane to be formed is positively charged. For this purpose, a lipid with quaternary amine has been employed as one of constitutional lipids, but the such lipid may show toxicity to cells to be transformed with the gene for expression. The invention selects, as constitutional lipids for liposomes, N-( alpha -trimethylammonioacetyl)-didodecyl-D-glutamate chloride (TMAG), dilauroylphosphatidylcholine (DLPC), and dioleoylphosphatidylethanolamine (DOPE) with molar ratio of 1 : 2 : 2, and vortex treatment for preparing the liposomes with multi-layers to entrap the gene therein.
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申请公布号 |
EP0475178(A1) |
申请公布日期 |
1992.03.18 |
申请号 |
EP19910114262 |
申请日期 |
1991.08.26 |
申请人 |
KABUSHIKI KAISHA VITAMIN KENKYUSHO |
发明人 |
YAGI, KUNIO;NODA, HITOSHI;OHISHI, NOBUKO;KURONO, MASAYASU |
分类号 |
C12N15/09;A61K9/127;A61K31/00;A61K48/00;A61P43/00;B01J13/02;C12N15/64 |
主分类号 |
C12N15/09 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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