发明名称 MESSENGER RNA STABILIZATION IN ANIMAL CELLS
摘要 of the Invention Disclosed is a method of increasing production of proteins, e.g., human tPA, in mammalian cells which normally secrete immunoglobulins. Degradation of mRNA transcribed from recombinant DNA is decreased by decreasing the length of the untranslated region of the mRNA. The untranslated region of DNA encoding a protein of interest is altered to produce a shorter recombinant DNA having an untranslated region comprising a poly A addition signal (AATAAA) and less than about 300 nucleotide bases interposed between said poly A signal and the stop codon 3' of the coding region of the gene of interest. The mammalian cell line is transfected and cultured to produce greater amounts of the protein of interest than the same cell line transfected with the unaltered DNA.
申请公布号 CA1297435(C) 申请公布日期 1992.03.17
申请号 CA19870545276 申请日期 1987.08.25
申请人 DAMON BIOTECH, INC. 发明人 GILLIES, STEPHEN D.
分类号 C12N15/09;C12N5/00;C12N5/10;C12N9/64;C12N9/72;C12N15/85;C12P21/00;C12P21/02;C12R1/91 主分类号 C12N15/09
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