NUCLEIC ACID FRACTIONATION BY COUNTER-MIGRATION CAPILLARY ELECTROPHORESIS

摘要

A method of fractionating nucleic acid fragments is disclosed. The method is based on counter migration of the fragments in an upstream direction through a polymer solution which is moving by electroosmotic flow in a downstream direction. Fractionation of selected-size nucleic acid fragments can be enhanced by reducing the difference between the electroosmotic flow rate and the migration rates of the selected-size fragments.