DNA probes for fingerpint analysis without tandem repeats

摘要

DNA probes for DNA fingerprint analysis. The DNA probes are 15 nucleotide sequences wherein 8 nucleotides of each sequence are G, 3 are T, 1 is C, 1 is A and 2 are A, C, G or T (N), except that the nucleotide sequence is not the M13 consensus sequence GAGGGTGGNGGNTCT. In particular, the 15-nucleotide sequence GGTGGNGATGGCTNG or as randomized variant of this sequence which is not the M13 consensus sequence, may be used as probes to detect hypervariable regions in genomic DNA. Methods for the preparation of such probes and for the detection of restriction length fragment polymorphisms using such probes are disclosed. The DNA probes are useful in genetic origin determination of human, animal or plant DNA-containing samples.