发明名称 |
GENERATION AND SELECTION OF NOVEL DNA-BINDING PROTEINS AND POLYPEPTIDES |
摘要 |
Novel DNA-binding proteins, especially repressors of gene expression, are obtained by variegation of genes encoding known binding protein and selection for proteins binding the desired target DNA sequence. A novel selection vector is used to reduce artifacts. Heterooligimeric proteins which bind to a target DNA sequence which need not be palindromic are obtained by a variety of methods, e.g., variegation to obtain proteins binding symmetrized forms of the half-targets and heterodimerization to obtain a protein binding the entire asymmetric target.
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申请公布号 |
US5096815(A) |
申请公布日期 |
1992.03.17 |
申请号 |
US19890293980 |
申请日期 |
1989.01.06 |
申请人 |
PROTEIN ENGINEERING CORPORATION |
发明人 |
LADNER, ROBERT C.;GUTERMAN, SONIA K.;KENT, RACHEL B.;LEY, ARTHUR C. |
分类号 |
C12N15/09;A61K38/00;C07K14/005;C07K14/195;C07K14/435;C07K14/81;C12N1/21;C12N15/10;C12N15/67;C12N15/70;C12N15/73;C12P21/00;C12R1/19;C40B40/02 |
主分类号 |
C12N15/09 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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