| 摘要 |
NEW MATERIAL:DNA fragment having sucrose phosphorylase gene having a restriction map shown by the formula (E is EcoRI; H is Hind III; R is RsaI; S is ScaI; Q is TaqI; T is EcoT141; V is EcoRV) and 1,800kd molecular weight. USE:For producing sucrose phosphorylase, etc. PREPARATION:Cells of culture mixture are collected from cultured cells of lactic acid bacteria Leuconostoc mesenteroides ATCC12,291, chromosome DNA is obtained and a chromosome DNA fragment mixture is obtained from a restriction enzyme to cause a projected end. A sucrose phosphorylase gene is obtained from DNA based on DNA mixed sequence estimated from N-end amino acid sequence of purified protein. |
