| 摘要 |
PURPOSE:To enable production of a phenylglycoside with a high production efficiency by transforming a gentianaceous plant cell with an Ri plasmid held by a bacterium of the genus Agrobacterium and then culturing the resultant hairy roots. CONSTITUTION:Apical buds or stem nodal parts are cut out of a gentianaceous plant body belonging to the genus Swertia, etc., and cultured. An Ri plasmid fragment held by a bacterium belonging to the genus Agrobacterium is then inoculated into a gentianaceous plant gene and cultured to develop hairy roots. Hairy roots having a high proliferation rate and many branches are selected from the resultant hairy roots, further cultured in a culture medium and proliferated in a large amount. The obtained hairy roots after completing the culture are extracted with an organic solvent such as ethanol or water. After distilling away the solvent, the residue is treated by column chromatography to recover the objective phenylglycoside. Thereby, since efficient production can be carried out, the aforementioned method is extremely suitable as an industrial method for producing the phenylglycoside. |
