| 摘要 |
<p>PURPOSE:To obtain the subject cell, having a lower average aggregation degree of cells and a smaller number of the maximum aggregate cells than those of BHK cells, and capable of producing stable and useful substances according to suspension culture in an eRDF culture medium by acclimatizing the BHK cells to a serum-free culture medium and the suspension culture. CONSTITUTION:BHK cells are transferred from a serum culture medium to a serum-free culture medium, acclimatized for 1 month, then cultured in a suspen sion culture vessel having a structure, equipped with a settling zone divided with a partition wall on the inside of an outer wall and capable of feeding oxygen with a cellular Teflon(R) tube for 19 days and further cloned. Thereby, the BHK cells are acclimatized to the serum-free culture medium and further to the suspension culture. As a result, the objective low aggregating cells having an aggregation degree of cells of <=80% based on that of the BHK cells and the number of the maximum aggregate cells of <=10% based on that of the BHK cells are obtained.</p> |
