发明名称 |
NUCLEAR HORMONE RECEPTOR-INTERACTING POLYPEPTIDES AND RELATED MOLECULES AND METHODS |
摘要 |
Disclosed is a method for determining whether a test protein is capable of interacting with a nuclear hormone receptor protein. The method involves: (a) providing a host cell which contains (i) a reporter gene operably linked to a protein binding site; (ii) a first fusion gene which expresses a first fusion protein, the first fusion protein including a nuclear hormone receptor protein covalently bonded to a binding moiety which is capable of specifically binding to the protein binding site; and (iii) a second fusion gene which expresses a second fusion protein, the second fusion protein including the test protein covalently bonded to a weak gene activating moiety; and (b) determining whether the test protein increases expression of the reporter gene as an indication of its ability to interact with the nuclear hormone receptor protein. Such an interaction may be hormone dependent, hormone independent, or hormone sensitive. |
申请公布号 |
WO9410338(A1) |
申请公布日期 |
1994.05.11 |
申请号 |
WO1993US10443 |
申请日期 |
1993.10.29 |
申请人 |
THE GENERAL HOSPITAL CORPORATION |
发明人 |
MOORE, DAVID, D.;LEE, JAE, WON |
分类号 |
G01N33/566;A61K38/00;A61P5/12;C07H21/04;C07K14/47;C07K14/72;C12N1/21;C12N5/10;C12N15/09;C12N15/10;C12N15/62;C12P21/02;C12Q1/02;C12Q1/68;(IPC1-7):C12Q1/00;A61K37/24;C07K13/00;A61K37/36;G01N33/53 |
主分类号 |
G01N33/566 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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