摘要 |
<p>PURPOSE:To provide the subject treating agent useful for producing useful substances such as cytokinin and not causing trouble such as elution by immobilizing N-glycolylneuraminic acid on the surface of an insoluble carrier with an amide bond. CONSTITUTION:An insoluble carrier (preferably having a thickness of >=100mu) such as a Schale, pipe, film or bottle made of the homopolymer, etc., of styrene or alpha-methylstyrene preferably after aminomethyl groups are introduced on the surface of the carrier is immersed in the solution of a mixture of Nglycolylneuraminic acid and a carbodiimide, etc., to immobilize the glycolylneuraminic acid on the carrier for providing the objective treating agent. Blood cells are cultured in the presence of the treating agent to produce cytokinin.</p> |